As the probe alignment error influences measurement results of tooth profile deviations of arc cylindrical worms （ZC worm） on the gear measuring center， a probe alignment error correction method should be established. Based on the equation of toroidal enveloping cylindrical worm （ZC1 worm） tooth flank and the probe alignment error， an axial profile measurement error model for the ZC1 worm is established to correct the axial coordinates of the tooth profile measurement points. Then， the worm axial profile deviations were evaluated according to the accuracy standards. The effects of varying thread number， module， and reference circle diameter on the tooth profile measurement error were analyzed. The experimental results show that the probe alignment error has little effect on the tooth profile form deviation， while the maximum difference between the total profile deviations before and after the probe alignment error correction reduced from 1.2 μm to 0.2 μm； that between the profile form deviation measurements reduced from 0.5 μm to 0.3 μm； and that between the profile slope deviations reduced from 2.5 to 0.4 μm. The proposed probe alignment error correction method can effectively reduce the effect of probe alignment errors on worm profile deviation measurements of gear measurement centers.