Laser & Optoelectronics Progress, Volume. 60, Issue 17, 1722001(2023)
A Straight-Axis Multi-Channel Optical Detection System for Nucleic Acid On-Site Detection
With the continuous demand for technological advances in nucleic acid detection for use in medical testing, point-of-care testing (POCT) has emerged as a research focus, presenting various challenges to overcome. To circumvent the disadvantages of POCT nucleic acid detection instruments that are currently in use, a straight-axis multi-channel optical detection system is proposed and designed, which facilitates both real-time, multi-channel as well as instant detection of nucleic acids . First, an optical path is built based on an orthogonal optical path design. Second, a new straight-axis multi-channel integration and switching device is designed to enable real-time switching between multiple fluorescence detection channels. Finally, the effectiveness of the optical path design is verified by simulation; this system is integrated into a self-designed polymerase chain reaction thermocycler, and a real-time fluorescence detection experiment is conducted using a new coronavirus standard. This system reaches a minimum coefficient of variation value of 0.02%. The minimum channel resolution is 0.49 mg/mL, and no channel crosstalk is observed. The system demonstrates good on-site detection capabilities, and functions cooperatively with the instrument to complete on-site detection from “sample in” to “result out”, offering a new perspective for POCT using multi-channel real-time fluorescence detection of nucleic acids.
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Bangchao Xi, Jiayu Yang, Shaolei Huang, Haozheng Dai, Juntian Zeng, Dongxu Zhang. A Straight-Axis Multi-Channel Optical Detection System for Nucleic Acid On-Site Detection[J]. Laser & Optoelectronics Progress, 2023, 60(17): 1722001
Category: Optical Design and Fabrication
Received: Aug. 3, 2022
Accepted: Sep. 15, 2022
Published Online: Sep. 13, 2023
The Author Email: Zhang Dongxu (zhangdongxu@xmu.edu.cn)