In this paper, datumobtained by multiwavelength UV-DIAL lidar at Anhui Institute of Optics and Fine Mechanicsare used to compare the effects of temperature and aerosol on singlewavelength (308 nm)and two-wavelength(308－355 nm) DIAL with backgr ound stratospheric aerosol. Results of twomethods show that the effects are larger for single-wavelength DIAL but less fortwo-wavelength DIAL. Calculated results of two methods are different distinctly. A meanvalue of 30 days show difference of ozone results measured by single-wavelength andtwowavelength is comparably large below 19 km altitude. Relative errors ofsingle-wavelength to two-wavelength are less than 12% from the height of 19 km to 35 km.