Journal of Innovative Optical Health Sciences, Volume. 16, Issue 1, 2330002(2023)
Observing single cells in whole organs with optical imaging
Cells are the basic unit of human organs that are not fully understood. The revolutionary advancements of optical imaging allowed us to observe single cells in whole organs, revealing the complicated composition of cells with spatial information. Therefore, in this review, we revisit the principles of optical contrast related to those biomolecules and the optical techniques that transform optical contrast into detectable optical signals. Then, we describe optical imaging to achieve three-dimensional spatial discrimination for biological tissues. Due to the milky appearance of tissues, the spatial information blurred deep in the whole organ. Fortunately, strategies developed in the last decade could circumvent this issue and lead us into a new era of investigation of the cells with their original spatial information.Cells are the basic unit of human organs that are not fully understood. The revolutionary advancements of optical imaging allowed us to observe single cells in whole organs, revealing the complicated composition of cells with spatial information. Therefore, in this review, we revisit the principles of optical contrast related to those biomolecules and the optical techniques that transform optical contrast into detectable optical signals. Then, we describe optical imaging to achieve three-dimensional spatial discrimination for biological tissues. Due to the milky appearance of tissues, the spatial information blurred deep in the whole organ. Fortunately, strategies developed in the last decade could circumvent this issue and lead us into a new era of investigation of the cells with their original spatial information.
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Xiaoquan Yang, Tao Jiang, Lirui Liu, Xiaojun Zhao, Ximiao Yu, Minjun Yang, Guangcai Liu, Qingming Luo. Observing single cells in whole organs with optical imaging[J]. Journal of Innovative Optical Health Sciences, 2023, 16(1): 2330002
Category: Research Articles
Received: Oct. 13, 2022
Accepted: Dec. 14, 2022
Published Online: Feb. 21, 2023
The Author Email: Luo Qingming (qluo@hainanu.edu.cn)