Spectroscopy and Spectral Analysis, Volume. 33, Issue 3, 686(2013)
Synchrotron Radiation-Based FTIR Microspectroscopy Study of 6-Hydroxydopamine Induced Parkinson’s Disease Cell Model
SH-SY5Y cell line treated with 6-hydroxydopamine (6-OHDA) is a classical Parkinson’s disease (PD). In the present study, synchrotron-based Fourier transform infrared (FTIR) microspectroscopy was used to analyze the biochemical composition of SH-SY5Y cell line treated with 6-OHDA. The detailed spectral analyses show the significant changes in cellular compositions such as lipids, proteins and nucleic acids in SH-SY5Y cells treated with 6-OHDA compared to control SH-SY5Y cells. As a result, the unsaturation levels of phospholipids decrease in SH-SY5Y cells treated with 6-OHDA compared to control cells, the analysis of protein secondary structure shows the significantly higher ratio of β-sheet in PD cells compared to that of control cells, and the content of nuclear acid is highly decreased compared to that of control cells, suggesting that 6-OHDA induces the serious oxidative damage in SH-SY5Y cells. These findings suggest that SR-FTIR is an effective and precise technical tool to probe the biochemical changes of cells and then evaluate the pathological damage in cells.
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ZHU Hong-yan, PEI Xiao, WU Ling-yan, QI Ze-ming, WANG Yu-yin, LIU Bo, ZHOU Hou-guang. Synchrotron Radiation-Based FTIR Microspectroscopy Study of 6-Hydroxydopamine Induced Parkinson’s Disease Cell Model[J]. Spectroscopy and Spectral Analysis, 2013, 33(3): 686
Received: Jul. 12, 2012
Accepted: --
Published Online: Mar. 27, 2013
The Author Email: Hong-yan ZHU (zhyred@shu.edu.cn)