Under the physiological condition that pH is 7.41, the mechanism of interactions between anthragallol (Ant) and deoxyribonucleic acid (DNA) is investigated by ultraviolet- visible spectroscopy and fluorescence spectroscopy using ethidium bromide (EB) as probe. The electrochemical characterization of Ant on glassy carbon electrode is determined by cyclic voltammetry. Based on the curves of cyclic voltammetry and the effect of EB on the interactions of Ant with DNA, it is proposed that the binding of Ant to DNA conforms to an intercalative mode. The results show that the binding ratio of Ant and DNA is 3.68:1, the binding constant (K) of Ant with DNA is 2.084×103 L/mol at 25 oC and the apparent molar absorption coefficient (e) is 2.21×104 L·mol-1·cm-1. The influence of salt effect on the interactions of Ant with DNA is studied, and the electrochemical changing rule of Ant is similar to EB with DNA. The results also show that Ant can bind with DNA, intercalation binding is the binding mode between Ant and DNA.