Optical Coherence Tomography (OCT) is a new kind of noninvasive cross-sectional imaging technique. In this paper, OCT system is implemented by use of superluminescent diodes and a tunable mode-locked Ti: Sapphire femtosecond pulse laser source. The properties of OCT system, transverse and axial resolution,are measured. The OCT image of plant and animal eye tissue are obtained. The quality of image with different light source is compared.