Measurement of atmosphere humidity profile is important for short time weather forecast and longtime climate analysis. There are several methods to measure atmosphere humidity profile, but they have disadvantages in different regions. Wind profiler radar has superiority in measuring humidity profile. The theory of wind profiler radar estimating atmosphere humidity profile is discussed. Based on the wind profile radar data and air sounding data of three timescales on 29, September 2009, the atmosphere humidity profile of the three timescale is estimated through calculating the atmosphere turbulent dissipation rating by using wind profiler radar and the temperature profile by using air sounding data. The result is reliable. Using wind profiler radar to estimate atmosphere humidity profile is feasible.