Multiphoton fluorescence microscopy is a powerful tool for studying biology. However, the current scanning method is not fast enough to fulfill the requirement for fast functional event detection in the neuroimaging field. A random-access scanning multiphoton fluorescence microscope system is reported. Two-dimensional acousto-optic deflectors (AODs) are used to scan rapidly the femtosecond excitation laser.The system is capable of achiering the scanning speed of 10 μs per scanning spot in the regin of thterest, and increases the effective scanning speed greatly. A single prism is used to compensate the severe dispersion of the excitation light introduced by the AODs. Using a 170 nm fluorescent bead, the lateral resolution of the system is measured to be 0.3 μm and the axial resolution is about 1.3 μm. Multiphoton fluorescence image of a fluorophore-labelled cell is acquired by using the random-access scanning system and a commercial multiphoton fluorescence microscope, respectively. The result proves the multiphoton fluorescence collection efficiency of the system.