Journal of Innovative Optical Health Sciences, Volume. 11, Issue 2, 1850003(2018)

Optical investigations reveal the effects of 2-aminoethyldiphenyl borate on STIM1 puncta formation

Tao Yu1... Shangbin Chen2, Jingying Pan3, Conglin Su3 and Jun He3,* |Show fewer author(s)
Author Affiliations
  • 1Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P. R. China
  • 2Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics — Huazhong University of Science and Technology, Wuhan, P. R. China
  • 3Division of Histology and Embryology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P. R. China
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    2-Aminoethyldiphenyl borate (2-APB) is the most commonly used pharmacological agent in the study of calcium release-activated channels (CRACs); however, its inhibitory mechanism to CRACs remains unclear. To address this issue, we systematically employed confocal imaging, dual-wavelength excitation photometry and FRET to examine the effects of 2-APB on the dynamic activities and function of STIM1 and Orai1, two key components of CRACs. Imaging results support that there are two signaling pathways (Orai1-independent and Orai1-dependent) for the formation of STIM1 puncta. 2-APB could dose dependently block Orai1-independent but not Orai1-dependent STIM1 puncta formation, despite its obvious inhibition effect on storeoperated Ca2+ entry (SOCE). In addition, we found that although 2-APB could not visibly alter near plasma membrane CAD-eYFP localization, it could completely block CAD-YFP-induced constitutive Ca2+ entry and promote the interaction between Orai1 and CAD by FRET measurements. Therefore, we proposed that inhibitory action of 2-APB on SOCE might attribute to its direct inhibitory effects on Orai1 channel itself, but not the interference on puncta formation between STIM1 and Orai1.

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    Tao Yu, Shangbin Chen, Jingying Pan, Conglin Su, Jun He. Optical investigations reveal the effects of 2-aminoethyldiphenyl borate on STIM1 puncta formation[J]. Journal of Innovative Optical Health Sciences, 2018, 11(2): 1850003

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    Paper Information

    Received: May. 22, 2017

    Accepted: Jul. 23, 2017

    Published Online: Sep. 18, 2018

    The Author Email: He Jun (junhe@mails.tjmu.edu.cn)

    DOI:10.1142/s1793545818500037

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