Acta Photonica Sinica, Volume. 44, Issue 10, 1017001(2015)

Method of Accurate Correction on Inner Filter Effects in Fluorescence Quenching Analysis

CHEN Xiang-dong*, YUAN Zi-jun, GAO Feng, and GAO Wei-qing
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    Via investigation on the evolution of fluorescence intensity in quenching process,it is discovered that the absorption distribution of exciting light can influence the fluorescence intensity in a potential way when the inner filter effects occur. According to the impact mechanism of absorption distribution on fluorescence intensity,a correction method based on fluorescence response function for removing the influence of absorption distribution is proposed. Photo induced fluorescence quenching of Quinquethiophene / fullerene (C70) at different excitation wavelength is tested to inspect the correction effect. Experimental results demonstrate these quenching efficiencies obtained without correction on the influence of absorption distribution weakly correlated with the absorption spectrum of C70,while the corrected ones are independent of them. Which means further correction on absorption distribution can effectively improve the final correction accuracy in fluorescence quenching analysis.

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    CHEN Xiang-dong, YUAN Zi-jun, GAO Feng, GAO Wei-qing. Method of Accurate Correction on Inner Filter Effects in Fluorescence Quenching Analysis[J]. Acta Photonica Sinica, 2015, 44(10): 1017001

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    Paper Information

    Received: Apr. 14, 2015

    Accepted: --

    Published Online: Nov. 30, 2015

    The Author Email: Xiang-dong CHEN (gdcxd@hfut.edu.cn)

    DOI:10.3788/gzxb20154410.1017001

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