Node location technology is the key of wireless sensor networks. Aiming at the low accuracy of the traditional centroid localization algorithms， a three-dimensional iterative centroid localization algorithm based on received signal strength indication is proposed. Based on the location technology， the algorithm simplifies the location reference nodes of unknown nodes， obtains the distance offset coefficient through the spatial geometric relationship between the nodes， uses the optimal solution of the coefficient to modify the centroid coordinate， and updates the coefficient repeatedly to update the location result until the iteration criterion is reached. The simulation results show that compared with the traditional centroid positioning algorithm， the proposed algorithm can improve the positioning accuracy by 20%-28%， and effectively reduce the positioning error of three-dimensional positioning in wireless sensor networks.