With the help of Surface-enhanced Raman scattering (SERS) technique, it is easy to acquire information on molecular structures that cannot be obtained by normal Raman spectroscopy, which overcomes the disadvantage of low sensitivity of Raman spectroscopy. Thus, it has become one of the most important methods for the trace detection of molecules and even single molecules, has been widely used in the life science, analytical chemistry and other fields. SERS substrate is the core component in SERS detection, however, only a few noble metal substrates have strong SERS effect, which are usually single-using, leading to the waste of resources in practical use. Herein, the history of SERS is reviewed, and the advance in the preparation of recyclable SERS substrates is highlightened. The discussion underscores the exciting prospect of developing the recyclable SERS substrates.