During the neurosurgery brain damage surgery, real-time monitoring of the target’s regional physiological parameters is very important. There isn’t a credible method on real-time evaluation rule at present. Some elementary research was carried out in this aspect. SD rats were chosen as experiment object. The target of treatment local of rat brain was damaged with radio-frequency, and minimally invasive near-infrared technology was used to measure hemoglobin oxygen saturation in situ on real-time. The changes in the process of radio-frequency damage were studied. The different changes of hemoglobin oxygen saturation were studied in reversible damage and irreversible damage. At the same time, some basic law was found on the analysis of a large number of experiment parameters. The results have some efficacy in certain reference value in future damage of stereotactic radio-frequency real-time assessment.