The Surface Plasmon Resonance (SPR) sensor is widely applied in the immune reaction detection of biomolecules.For the self-developed angle scanning surface plasmon resonance sensor,the kinetics detection and kinetics data analysis software system were developed according to Langumair model,and the data processing method of polynomial fitting combined with centroid method was proposed.The established method could effectively inhibit the interference on SPR resonance angle detection by noise,ambient temperature and mechanical vibration in the process of detection.The immune reaction kinetics of mice antigen and antibody was detected.Its association rate constant ka≈2.05×104 M-s- and issociation rate constant kd≈0.005 s-.The feasibility of the established device and data processing method was confirmed.This method could effectively improve the accuracy and capacity of resisting disturbance of angle scanning surface plasmon resonance sensor,and it is worthy to be applied and promoted.