Journal of Atmospheric and Environmental Optics, Volume. 13, Issue 2, 97(2018)
Design of Chlorophyll Fluorescence Detection System Based on m Sequence Modulation Technique
According to the problems in the detection of weak chlorophyll fluorescence, a chlorophyll fluorescence detection system based on m sequence modulation technology was proposed. On the basis of noise analysis of fluorescence detection circuit, I/V conversion circuit and secondary amplifier circuit that based on T-type resistance feedback network were designed. According to the principle of m sequence demodulation, a bipolar conversion circuit, a phase-shift circuit and a fluorescence signal demodulation circuit based on AD630 were designed. The system was applied to measure the chlorophyll fluorescence of chlorella pyrenoidosa. For a sample with a chlorophyll concentration of 10 μg/L, the relative standard deviation of measurement values was 2.61%. The linear correlation coefficient between the output DC voltage and chlorophyll concentration was 0.998 in a chlorophyll concentration range of 0~100 μg/L.
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SHI Chaoyi, GAO Xianhe, LU Jun, GU Yanhong. Design of Chlorophyll Fluorescence Detection System Based on m Sequence Modulation Technique[J]. Journal of Atmospheric and Environmental Optics, 2018, 13(2): 97
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Received: Jan. 29, 2018
Accepted: --
Published Online: Apr. 23, 2018
The Author Email: Xianhe GAO (gaoxh@hfuu.edu.cn)