For enhancing the 2.0 μm band fluorescence of Ho³⁺, a certain amount of WO³oxide was introduced into Ho³⁺/Tm³⁺/Yb³⁺tri-doped tellurite glass prepared using melt-quenching technique. The prepared tri-doped tellurite glass was characterized by the absorption spectra, fluorescence emission and Raman scattering spectra, together with the stimulated absorption, emission cross-sections and gain coefficient. The research results show that the introduction of WO³oxide can further improve the 2.0 μm band fluorescence emission through the enhanced phonon-assisted energy transfers between Ho³⁺/Tm³⁺/Yb³⁺ions under the excitation of 980 nm laser diode (LD). Meanwhile, the maximum gain coefficient of Ho³⁺at 2.0 μm band reaches about 2.36 cm^-1. An intense 2.0 μm fluorescence emission can be realized .