The technology of fluorescence excited by ultraviolet is used for the study of fluorescence spec. trum and quantitative methods of nitrocellulose. Firstly, fluorescence analysis process is implemented on the nitro. cellulose in the solution of acetone. The fluorescence spectrum of nitrocellulose excited by ultraviolet is obtained through three-dimensional scanning technology. So the wavelength of excitation and emission are 355.0 nm and 354.5 nm respectively at the maximum fluorescence relative intensity. Then the fluorescence relative intensities of nitrocellulose in acetone with different concentrations are measured under the condition of 354.5 nm emission wavelength. So the quantitative method of nitrocellulose is determined through the linear regress of the experi. ment result.