The present paper studied fluorescence fingerprint properties of the municipal wastewater with industrial wastewater as major components. There existed three typical fluorescence peaks in the excitation-emission matrix of the municipal wastewater, locating at about λex/λem of 275/310, 230/340 and 220/310 nm respectively. The wastewater didn’t display typical protein-like fluorescence as the municipal wastewater with domestic sewage as major component. The fluorescence intensity of the wastewater was quite high with remarkable difference between workday and weekend. These might relate to the high content of industrial wastewater. The advantages of the fluorescence fingerprint such as easy and fast measurement and rich information about the components of wastewater make it a novel tool in water quality monitoring and early-warning.