In the comprehensive experiment of applied chemistry by adopting the offline experimental teaching mode, there are some shortcomings, such as limited preview resources, single teaching form, insufficient demonstration of experimental operation, and students’ passive learning initiative. To solve these problems, taking the comprehensive experiment of “preparation of defective bismuth tungstate by solvothermal method and its photocatalytic performance test” as an example, the online and offline mixed experimental teaching mode is implemented in the applied chemistry major for the first time. This teaching mode can fully mobilize students’ learning initiative, and provide useful reference for the reform of experimental teaching.