A new calibration method based on the spherical model for fisheye lens using two- dimensionally calibration target is presented. According to the relationship between the point on arc and the image points for the spherical model, the internal parameters by the points in a straight line on the plane target are preliminary estimate. And the corresponding external parameters by the mapping between the world points and the points in arc on the sphere are calculated. The rooy- mean- square error of the image points and the reprojection points are taken as the optimization parameter and the exact solution of the internal parameters, the distortion coefficients and the external parameters are solved by the nonlinear optimization algorithm. The experiment presents that the proposed method can quickly estimate the initial values of the internal and external parameters for the fisheye lens with high-precision and meet the practical applications.