Journal of Innovative Optical Health Sciences, Volume. 10, Issue 5, 1743006(2017)

Three-dimensional, isotropic imaging of mouse brain using multi-view deconvolution light sheet microscopy

Sa Liu1, Jun Nie1, Yusha Li2, Tingting Yu2, Dan Zhu2、*, and Peng Fei1,2
Author Affiliations
  • 1School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan 430074, P. R. China
  • 2Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, P. R. China
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    We present a three-dimensional (3D) isotropic imaging of mouse brain using light-sheet fluorescent microscopy (LSFM) in conjunction with a multi-view imaging computation. Unlike common single view LSFM is used for mouse brain imaging, the brain tissue is 3D imaged under eight views in our study, by a home-built selective plane illumination microscopy (SPIM). An output image containing complete structural information as well as significantly improved resolution (~4 times) are then computed based on these eight views of data, using a bead-guided multi-view registration and deconvolution. With superior imaging quality, the astrocyte and pyramidal neurons together with their subcellular nerve fibers can be clearly visualized and segmented. With further including other computational methods, this study can be potentially scaled up to map the connectome of whole mouse brain with a simple light-sheet microscope.

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    Sa Liu, Jun Nie, Yusha Li, Tingting Yu, Dan Zhu, Peng Fei. Three-dimensional, isotropic imaging of mouse brain using multi-view deconvolution light sheet microscopy[J]. Journal of Innovative Optical Health Sciences, 2017, 10(5): 1743006

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    Paper Information

    Received: Jul. 29, 2017

    Accepted: Aug. 28, 2017

    Published Online: Jan. 10, 2019

    The Author Email: Zhu Dan (dawnzh@mail.hust.edu.cn)

    DOI:10.1142/s1793545817430064

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