Advanced Photonics, Volume. 3, Issue 4, 044001(2021)
Review of bio-optical imaging systems with a high space-bandwidth product
Jongchan Park1, David J. Brady2, Guoan Zheng3,4, Lei Tian5, and Liang Gao1,*
Author Affiliations
1University of California, Department of Bioengineering, Los Angeles, California, United States2University of Arizona, James C. Wyant College of Optical Sciences, Tucson, Arizona, United States3University of Connecticut, Department of Biomedical Engineering, Storrs, Connecticut, United States4University of Connecticut, Department of Electrical and Computer Engineering, Storrs, Connecticut, United States5Boston University, Department of Electrical and Computer Engineering, Boston, Massachusetts, United Statesshow less
Optical imaging has served as a primary method to collect information about biosystems across scales—from functionalities of tissues to morphological structures of cells and even at biomolecular levels. However, to adequately characterize a complex biosystem, an imaging system with a number of resolvable points, referred to as a space-bandwidth product (SBP), in excess of one billion is typically needed. Since a gigapixel-scale far exceeds the capacity of current optical imagers, compromises must be made to obtain either a low spatial resolution or a narrow field-of-view (FOV). The problem originates from constituent refractive optics—the larger the aperture, the more challenging the correction of lens aberrations. Therefore, it is impractical for a conventional optical imaging system to achieve an SBP over hundreds of millions. To address this unmet need, a variety of high-SBP imagers have emerged over the past decade, enabling an unprecedented resolution and FOV beyond the limit of conventional optics. We provide a comprehensive survey of high-SBP imaging techniques, exploring their underlying principles and applications in bioimaging.