Spectroscopy and Spectral Analysis, Volume. 30, Issue 9, 2550(2010)
Effect of Heat Treatment on the Antigenicity and Conformation of Peanut Allergen Ara h 2
Peanut allergen Ara h 2 was extracted from peanuts and was identified by SDS-PAGE and MALDI-TOF-MS. Effect of heat treatment on the antigenicity and structure of Ara h 2 was measured by indirect ELISA, CD, fluorescence and UV absorption spectra. The results showed that the antigenicity of Ara h 2 had a slight increase after being heated at 55 or 70 ℃, while above 85 ℃ the antigenicity decreased significantly, and the antigeicity of Ara h 2 decreased with increasing temperature. The CD showed that the secondary structure of Ara h 2 was changed after heat treatment. The ANS fluorescence probe emission spectra analysis demonstrated that the heat treatment induced an increase in surface hydrophobicity of Ara h 2. The UV absorption spectra showed that the absorption maximum wavelength was increased when Ara h 2 was heated except the sample heating at 50 ℃ for 30 min. So the changes in conformation of Ara h 2 lead to the antigenicity degression.
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HU Chun-qiu, GAO Jin-yan, CHEN Hong-bing, LUO Chun-ping, YAN Fei. Effect of Heat Treatment on the Antigenicity and Conformation of Peanut Allergen Ara h 2[J]. Spectroscopy and Spectral Analysis, 2010, 30(9): 2550