It is necessary to improve the anti-electromagnetic interference ability of unmanned surface vehicle(USV) navigation; one method is to apply polarization sensors in fluctuant water environments and formation coordination.In this study, an integrated navigation system was designed based on a polarization sensor, micro inertial measurement unit (MIMU), and global positioning system (GPS). A gimbal was mounted for the polarization sensor, and a USV experimental platform was built for navigation and formation experiments. First, the principle of polarized light navigation and USV formation were introduced.Subsequently, the integrated navigation system with a polarization sensor was designed based on Kalman filtering. Finally, tracking and formation of USV experiments were performed based on the integrated navigation system. The results of the tracking experiment show that the heading angle error and position error of the polarization sensor/MIMU/GPS integrated navigation system are 6.055° and 0.209 m, respectively.The polarized light integrated navigation system could work normally even if the magnetic compass was disturbed. The results of the formation experiment show that the leader tracking error is 0.425 m, and the follower formation error is 0.707 m. The polarization sensor can be used in fluctuant water circumstance navigation, and the polarized light integrated navigation system can be used in USV navigation and formation.