Journal of Innovative Optical Health Sciences, Volume. 12, Issue 6, 1950023(2019)
Super-resolution microscopy based on parallel detection
Image scanning microscopy based on pixel reassignment can improve the confocal resolution limit without losing the image signal-to-noise ratio (SNR) greatly [C. J. R. Sheppard, "Super-resolution in confocal imaging," Optik 80(2) 53–54 (1988). C. B. Müller, E. Jorg, "Image scanning microscopy, "Phys. Rev. Lett. 104(19) 198101 (2010). C. J. R. Sheppard, S. B. Mehta, R. Heintzmann, "Superresolution by image scanning microscopy using pixel reassignment," Opt. Lett. 38(15) 2889–2892 (2013)]. Here, we use a tailor-made optical fiber and 19 avalanche photodiodes (APDs) as parallel detectors to upgrade our existing confocal microscopy, termed as parallel-detection super-resolution (PDSR) microscopy. In order to obtain the correct shift value, we use the normalized 2D cross correlation to calculate the shifting value of each image. We characterized our system performance by imaging fluorescence beads and applied this system to observing the 3D structure of biological specimen.
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Zhimin Zhang, Shaocong Liu, Liang Xu, Yubing Han, Cuifang Kuang, Yong Liu, Xiang Hao, Hongqin Yang, Xu Liu. Super-resolution microscopy based on parallel detection[J]. Journal of Innovative Optical Health Sciences, 2019, 12(6): 1950023
Received: Jan. 11, 2019
Accepted: --
Published Online: --
The Author Email: Kuang Cuifang (cfkuang@zju.edu.cn)