Laser & Optoelectronics Progress, Volume. 60, Issue 11, 1106012(2023)
Research Progress of Super-Resolution Fluorescence Microscopy
Super-resolution fluorescence microscopy (SRFM), which can bypass the optical diffraction limit, provide an extremely important research tool for investigations of the subcellular structures and the dynamic processes of biological macromolecules. Therefore, since its invention, SRFM has been paid attention to and applied by scientists in the field of biology and medicine. Benefiting from the rapid development of life science and biotechnology in recent years, SRFM has been developed unprecedentedly. In this paper, we will mainly focus on the research progresses of four new kinds of SRFM, including minimal photon fluxes (MINFLUX), super-resolution optical fluctuation imaging (SOFI), super-resolution image based on anti-bunching effect, and deep-learning based super-resolution microscopy, respectively. We will briefly describe the recent research progresses and applications of these SRFM techniques from the basic principles, experimental implementation methods and related requirements, imaging performance, and comparison with other technologies, as well as the combination with other super-resolution techniques, to provide some references for researchers in this field.
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Aoni Wei, Chengbing Qin, Shuai Dong, Xinqin Meng, Yunrui Song, Xiangdong Li, Xilong Liang, Guofeng Zhang, Ruiyun Chen, Jianyong Hu, Zhichun Yang, Jianzhong Huo, Liantuan Xiao, Suotang Jia. Research Progress of Super-Resolution Fluorescence Microscopy[J]. Laser & Optoelectronics Progress, 2023, 60(11): 1106012
Category: Fiber Optics and Optical Communications
Received: Mar. 1, 2023
Accepted: Apr. 4, 2023
Published Online: Jun. 7, 2023
The Author Email: Qin Chengbing (chbqin@sxu.edu.cn), Xiao Liantuan (xlt@sxu.edu.cn)