Journal of Innovative Optical Health Sciences, Volume. 7, Issue 2, 1350041(2014)
Intracellular oxygen: Similar results from two methods of measurement using phosphorescent nanoparticles
The ability to resolve the spatio-temporal complexity of intracellular O2 distribution is the \Holy Grail" of cellular physiology. In an effort to obtain a minimally invasive approach to the mapping of intracellular O2 tensions, two methods of phosphorescent lifetime imaging microscopy were compared in the current study and gave similar results. These were two-photon confocal laser scanning microscopy with pinhole shifting, and picosecond time-resolved epi-phosphorescence microscopy using a single 0.5 μm focused spot. Both methods utilized Ru coordination complex embedded nanoparticles (45 nm diameter) as the phosphorescent probe, excited using pulsed outputs of a titanium–sapphire Tsunami lasers (710–1050 nm).
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David Lloyd, Catrin F. Williams, K. Vijayalakshmi, M. Kombrabail, Nick White, Anthony J. Hayes, Miguel A. Aon, G. Krishnamoorthy. Intracellular oxygen: Similar results from two methods of measurement using phosphorescent nanoparticles[J]. Journal of Innovative Optical Health Sciences, 2014, 7(2): 1350041
Received: Jun. 30, 2013
Accepted: Sep. 1, 2013
Published Online: Jan. 10, 2019
The Author Email: Lloyd David (lloydd@cf.ac.uk)