To obtain pL class adhesive drop to match the micro parts in size, three kinds of micro drop making mechanisms, injection, bubble jetting, and needle transfer,were analyzed. A pL class adhesive dispensing approach based on time-pressure dispensing was present,then pL class adhesive spots were obtained by online visual monitoring of the spot diameter, and controlling the internal diameter of the dispensing needle tip, pressure and time. The effects of three factors mentioned above on the adhesive spot size were experimented, and this technique combined with a micromanipulation system were used in Inertial Confinement Fosion(ICF) experiments to bond a fill tube and a capsule together for the cryogenic targets. The results show that the adhesive spot diameter is proportional to the internal diameter of the dispensing needle tip, pressure and time. When the internal diameter of needle tip,pressure and time are controlled to be 1.2 μm, 0 psi, and 8~10 s,the adhesive volume and diameter will be less than 3pL and 40 μm, respectively.