A Doppler frequency shift method based on acousto-optic modulation for microwave signals was introduced in this paper. This technology is based on microwave photonics signal processing and acousto-optic frequency shifting technology. The Doppler frequency shift of microwave signals in the optical domain has been realized and it can be used for generating radar interference signals. The operating frequency range of the system is 2-20 GHz. The test results show that the frequency shift range of the velocity deception interference signals is ±1 MHz and the minimum frequency shift is ± 10 Hz. The system realizes the Doppler frequency shift simulation test of radar pulse signal when the target velocity reduced from 1 200 m/s to 1 000 m/s.