An analytical method for the accurate determination of 12 kinds of trace elements in wild Artemisia Selengensis by inductively coupled plasma tandem mass spectrometry (ICP-MS/MS) was established. In the MS/MS mode, AsO and SeO were formed by mass shift reaction of As and Se with O2, and AsO and SeO were used to eliminate mass spectral interferences. Using NH3/He as the reaction gas, Cr, Mn, Fe, Co, Ni, Cu, and Zn were reacted with NH3/He to form clusters ions, by measuring the cluster ions to eliminate mass spectral interferences. Cd, Hg and Pb were measured using standard mode. The calibration curves of analytes in the 0～200 μg·L-1 range has a good linear relationship, and the detection limit is 0.64～49.61 ng·L-1. By analyzing the national standard material celery (GBW 10048), the difference test was carried out by t test, and the results showed that there was no significant difference between the measured value and the certified value, and the method was proved to be accurate and reliable. The spike recovery was 92.0%～106.1%, and the RSD was 1.6%～4.9% for twelve elements. Analyzing from 4 samples of wild Artemisia Selengensis in different areas of China, according to the results, different sources of 12 elements content in the samples vary, the contents of Mn, Fe, Zn are much higher than the rest of the 9 kinds of elements, and poison rational elements of As, Cd, Hg, Pb content are very low. The method can accurately determine a variety of trace elements in the wild Artemisia Selengensis, and provide scientific theoretical basis for the edible nutrition and safety of the wild Artemisia Selengensis.