Based on global vegetation fluorescence distribution, photon path probability distribution density function factor and the fluorescence intensity at 755 nm are synchronously inverted with GOSAT data. The inversion results are compared with the results of TCCON site. The results show that for the GOSAT data near the Park Falls (45.9°N, 90.3°W) site, which is greatly affected by plant chlorophyll fluorescence, the maximum deviation between CO2 inversion results before and after considering the effect of fluorescence is 1.6×10 -6. While for the GOSAT data of near Sodankyla site which is slightly affected by fluorescence, the maximum deviation is 0.8×10 -6. The scattering correction of the fluorescence effect can reduce the average error to about 0.1×10 -6.