Journal of Innovative Optical Health Sciences, Volume. 16, Issue 4, 2350017(2023)

A simple Bessel module with tunable focal depth and constant resolution for commercial two-photon microscope

Ting Mo1,¶... Yiran Liu2,¶, Fatao Bie1, Zimin Dai1, Jin Chang1, Hui Gong1,2,3, and Wei Zhou1,23,* |Show fewer author(s)
Author Affiliations
  • 1Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, P. R. China
  • 2MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei 430074, P. R. China
  • 3Research Unit of Multimodal Cross Scale, Neural Signal Detection and Imaging, Chinese Academy of Medical Sciences, HUST-Suzhou Institute for Brainsmatics, JITRI Suzhou, P. R. China
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    The volumetric imaging of two-photon microscopy expands the focal depth and improves the throughput, which has unparalleled superiority for three-dimension samples, especially in neuroscience. However, emerging in volumetric imaging is still largely customized, which limits the integration with commercial two-photon systems. Here, we analyzed the key parameters that modulate the focal depth and lateral resolution of polarized annular imaging and proposed a volumetric imaging module that can be directly integrated into commercial two-photon systems using conventional optical elements. This design incorporates the beam diameter adjustment settings of commercial two-photon systems, allowing flexibility to adjust the depth of focus while maintaining the same lateral resolution. Further, the depth range and lateral resolution of the design were verified, and the imaging throughput was demonstrated by an increase in the number of imaging neurons in the awake mouse cerebral cortex.

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    Ting Mo, Yiran Liu, Fatao Bie, Zimin Dai, Jin Chang, Hui Gong, Wei Zhou. A simple Bessel module with tunable focal depth and constant resolution for commercial two-photon microscope[J]. Journal of Innovative Optical Health Sciences, 2023, 16(4): 2350017

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    Paper Information

    Category: Research Articles

    Received: Mar. 24, 2023

    Accepted: May. 25, 2023

    Published Online: Jul. 28, 2023

    The Author Email: Zhou Wei (wzhou@mail.hust.edu.cn)

    DOI:10.1142/S1793545823500177

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