Laser & Optoelectronics Progress, Volume. 55, Issue 10, 101702(2018)
High Resolution Melting Technology Based on Fabry-Perot Microcavity Laser
We study a high-resolution melting (HRM) analysis method based on laser signal in self-made Fabry-Perot (FP) optical microcavity with high quality. We utilize the F-P optical microcavity as the micro-laser cavity, intercalated saturation dyes as the gain media and emitted laser signal as the detection signal to achieve high-resolution detection and screening of mismatched DNA by temperature scanning. We investigate on the melting curves of target DNA and mismatched DNA with 25 base-pairs and 50 base-pairs, respectively. Theoretical and experimental results indicate that the HRM detection method based on laser signal has lower melting temperature and higher signal-to-noise ratio.
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Liang Xiyue, Hou Mengdi, Zhang Tingting, Qiu Chengyu, Wang Wenjie. High Resolution Melting Technology Based on Fabry-Perot Microcavity Laser[J]. Laser & Optoelectronics Progress, 2018, 55(10): 101702
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Received: Apr. 10, 2018
Accepted: --
Published Online: Oct. 14, 2018
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