The unconsciousshake of human eye leads to the random variations of image position, and makes it impossible to observe a specific area. To solve the problem, during the object imaging, a real time “calculationmovement”stabilizing image algorithm was presented based on image matching theory. It uses a series of continuously grabbed images that focus on nearly the same area of retina. Therefore, it is possible to find the center area of the image through “samplingcalculaiton”, and do the correlation between the center area and that of the first image so as to get the direction and amplitude of movement, finally move the picture according the results. Repeat this process to stabilize the series of images and remove those unmatched images, which make the object image nearly on the same position. The results indicates that the algorithm can overcome the random variation problem of object image position and stabilize the retinal image in real time for the adaptive optics imaging setup; overcome the problem that one cannot focus on the same area for a long time because of laser′s strongly irritation, and realize the retina and blood capillary observation with the same setup and same algorithm; make it possible to get anpatched image with large field of view.