Journal of Synthetic Crystals, Volume. 49, Issue 3, 547(2020)
Preparation and Properties of Gelatin/Biphasic Calcium Phosphate Porous Ceramic Scaffold
Hydroxyapatite(HAP) is similar to the inorganic components of human bones, and has good biocompatibility and bone conduction properties. Β-Tricalcium Phosphate (β-TCP) is a biodegradable and bioabsorbable bioactive material. Its degradation products Ca, P can enter the living body's circulatory system to form new bone, becoming an ideal hard tissue replacement material. Calcium carbonate (CaCO3) and phosphoric acid (H3PO4) were used as raw materials to synthesize biphasic calcium phosphate ceramic powder by direct precipitation method. Then, polyurethane foam carrier was prepared by laser forming technique, and porous gelatin/biphasic calcium phosphate ceramic scaffold was prepared by a foam dipping method. Finally, glutaraldehyde is used to crosslink with the scaffold to improve performance. The composition, morphology and structural characteristics of the composite porous scaffolds were analyzed by XRD and SEM. The degradability, porosity, mechanical properties and cytotoxicity of the composite bioscaffolds were evaluated. The results show that the powder prepared by the experiment was biphasic calcium phosphate, and the composition includes β-Tricalcium Phosphate (β-TCP) and Hydroxyapatite (HAP), among them, β-TCP is the main phase. The biological scaffold has a good pore structure, including a regular through hole and an irregular communication hole, the through hole diameter is between 800 μm and 950 μm, the irregular communication hole is between 300 μm and 500 μm, and the average porosity of the scaffold is 83.1%; The average compressive strength of the scaffold reaches 1.06 MPa, which satisfies the mechanical requirements of the bone tissue engineering for the scaffold material; the cytotoxicity of the bioscaffold is grade 0 or grade 1, no cytotoxicity, and has good degradation performance.
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LI Kexin, GUO Huachao, LI Pan, JIANG Zhimin, YAN Tingting. Preparation and Properties of Gelatin/Biphasic Calcium Phosphate Porous Ceramic Scaffold[J]. Journal of Synthetic Crystals, 2020, 49(3): 547
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Received: --
Accepted: --
Published Online: Jun. 15, 2020
The Author Email: Tingting YAN (itty@foxmail.com)
CSTR:32186.14.