Cold brown calcic soils, the major farmland soil type, in the middle reach of the Yarlung Zangbo River valley was selected as the experimental object, and then the main experimental items of soil and fertilizer sciences were systematically integrated to design a comprehensive experiment. Through the scientific design of comprehensive experiment, the students can not only master the determination methods, grading standards and relationship of the main physical and chemical indexes of soil, but also discuss the agricultural production characteristics of cold brown calcium soil according to the result of soil samples, so as to serve the local agricultural production practice. This comprehensive experiment design combined education, research and production practice, which not only trained the students’ experimental operation skills, but also improved their comprehensive analytical ability, and achieved good experimental teaching effect.