Cu-In-Zn-S (CIZS) quaternary quantum dots (QDs) were prepared by hydrothermal method. The effects at different reaction temperatures and Cu/In molar ratios on phase composition, microscopic morphology, and fluorescence performance of CIZS QDs were studied by X-ray diffraction (XRD), energy spectrometer (EDS), transmission electron microscope (TEM), and fluorescence spectrophotometer (PL). The surface property of CIZS QDs was characterized by Fourier transform infrared spectrometer (FT-IR). The results show that the QDs presented spherical shape with good dispersion in aqueous solution, and the particle sizes were 3-4 nm. Moreover, the prepared CIZS QDs exhibites excellent luminescence property. The emission intensity of CIZS QDs was gradually improved with the increase of reaction temperature, and the QDs exhibited the strongest emission at 110 ℃. However, the impurity of In2S3 phase was formed at excessive temperature, resulting in the decrease of emission intensity. Moreover, the emission peak of CIZS QDs show a blue-shift tendency from 675 nm to 644 nm with the decrease of Cu/In ratios, and the emission intensity gradually enhanced and reached maximum value at n(Cu)/n(In)=1∶7. Meanwhile, the quantum yield peaked at 6.2%. The LED based on CIZS quantum dot has achieved luminescence successfully, CRI values of 81.2 and LE of 36.8 lm/W, which indicated that CIZS QDs have a good application prospect in the field of lighting.