Aiming at the problem of large errors in the calculation of the deflection angle of the substrate and the position of the pixel pit in the display ink printing of e-paper， a high-precision substrate angle correction and location method was proposed. Firstly， a vision detection system was constructed according to the requirements of inkjet printing， which was used for high-resolution imaging of substrate markings. The installation angle calibration and multi-camera relative position calibration of the vision system were carried out by improving the camera's intrinsic and extrinsic parameter calibration methods and improving the microscopic imaging calibration method. Then， edge detection and contour moment feature calculation were performed on the substrate markings， the center pixel coordinates of the markings were extracted， and high-precision substrate correction was achieved using coordinate transformation and least squares line fitting. Finally， the initial pixel pit positioning was completed by indirectly using the substrate alignment marks. Experimental results show that the correction error of the substrate angle correction algorithm is within ±2″， and the pixel pit positioning error is within ±3 μm， far exceeding the accuracy requirements of ink filling. By establishing a vision detection system and designing substrate correction and positioning algorithms， high-precision and efficient printing filling of display ink were achieved， improving the yield of printing equipment， which is of great guiding significance for the localization of display ink printing and filling equipment.