Acta Optica Sinica, Volume. 37, Issue 3, 318002(2017)
Structure-Guided Development of Reversibly Photoswitchable Green Fluorescent Proteins Probe
In recent years, people pay more and more attentions to the development of reversibly photoswitchable fluorescent proteins (RSFPs), because those fluorescent proteins can greatly promote the development and application of live-cell super-resolution imaging techniques. Reversibly photoswitchable fluorescent protein can be repeatedly and reversibly modulated by different wavelength lights, thus it can be widely applied in many fields such as high density data optical storage, the measurement of photochromic fluorescence resonance energy transfer, and super-resolution imaging based on reversible saturable linear fluorescence transitions (RESOLFT) principle. The recent development of green RSFPs is reviewed from the key amino acids involved in RSFPs design. We also make a brief discussion of the relationship between structure and optical characteristics of RSFPs, serving as the reference for further structure-guided development of better RSFPs.
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Wang Sheng, Chen Xuanze, Chang Lei, Xue Ruiying, Sun Yujie. Structure-Guided Development of Reversibly Photoswitchable Green Fluorescent Proteins Probe[J]. Acta Optica Sinica, 2017, 37(3): 318002
Received: Oct. 31, 2016
Accepted: --
Published Online: Mar. 8, 2017
The Author Email: Sheng Wang (wangsheng9411@sina.com)