The measurement of blood glucose are enzymatic and biochemical methods which are destructive. Raman technique is used to detect blood glucose and a new data analysis method is established to analyse blood glucose concentration, which is a noninvasive and rapid blood glucose tests. Using mice as experimental models, Raman spectra of blood taken from the tails are acquried half an hour after injection of glucose. Raman spectra are collected every 15 minutes and accompanied by blood glucose meter to get the blood glucose value to be reference. 1 125 cm－1 is characteristic peak of glucose and glucose in blood is called blood glucose, so 1 125 cm－1 is chosen as the peak of blood glucose in the Raman spectra of blood. 1 549 cm－1 is characteristic peak of hemoglobin which is stable in the body, so 1 549 cm－1is used to be internal standard to research 1 125 cm－1 intensity. The result shows that the change of 1 125 cm－1/1 549 cm－1 may well correspond with changes in blood glucose and they have a good linear relationship. The experiments show that this Raman technique can reflect the changes of blood sugar levels noninvasive.