The method of pose measurement using computer vision is widely used in modern control, navigation, tracking and other fields. A monocular visual pose measurement method was studied and designd based on P4P rectangular distribution of planar target and EPNP algorithm in this paper. Firstly, a single camera was used to obtain the plane target image. After the image processing, the pixel coordinates of the four feature points were obtained, and the EPNP algorithm was used to perform the attitude calculation. Secondly, the simulation analysis of pose error measurement provides theoretical guidance and basis for improving attitude measurement accuracy. Finally, a coordinate system registration method combined with a high-precision two-dimensional turntable was proposed. The method was used to verify the accuracy of the three-direction attitude angles. The experimental results show that the rotation angle around the x and y axes is [-6°, 6°], the pose measurement error is less than 0.1° to meet the measurement application requirements.