A newly-developed six-degree-of-freedom calibration system for the wind tunnel balances is introduced. The frame of the system, the functions and the operating principle of different parts are presented in detail. The system is composed of four parts: the automatically loading subsystem, the automatically resetting subsystem, the data-acquisition subsystem and the measurement subsystem. The results of some cell experiments proved that the system can meet the needs of the present calibration task of the balance. Through further improvement, the system can be also used to calibrate other devices with multi degree-of-freedom and measure the minute shifts, such as the guide rail of machine tool and the assembling of large parts and so on.