Concentration and temperature measurement are of great significance for engine design, CFD model establishment and numerical simulation verification. Planar laser-induced fluorescence (PLIF) technology has the advantages of simple experimentation, object selectivity and high measurement sensitivity. Among them, the OH-PLIF measurement of concentration and temperature is more widely applied due to their theoretical maturity and technical convenience. However, the application of OH-PLIF in the kerosene combustion field is limited because of the great interference of residual kerosene. This paper focus on the kerosene interference problem in the measurement of OH fluorescence distribution. The absorption and fluorescence emission spectra of OH and kerosene were comparative analysis. The absorption spectrum of kerosene vapor is obtained from the intensity ratio of the strontium halogen lamp before and after its pass through the kerosene vapor. Compared with the isolated absorption line of OH in the 260~320 nm band, the kerosene absorption is a wide absorption band from 240 to 320 nm. The kerosene absorption band completely covers the OH excitation line. When the OH is excited in this band in kerosene combustion, it is inevitable to stimulate the kerosene to produce fluorescence. On the other hand, kerosene fluorescence and the OH/kerosene mixed fluorescence were measured by adjusting the excitation wavelength. The kerosene fluorescence emission centered at 290 and 340 nm, while OH fluorescence is mainly concentrated at wavelength 300~320 nm. Combined with the absorption spectrum, kerosene fluorescence covers OH fluorescence indicating that the OH measurement in the 280 nm band can not be carried out by a single frequency domain filter when there are residua. In this paper, the kerosene interference was eliminated by measuring kerosene simultaneously and deducted from mixed fluorescence then. The fluorescence was detected selectively by adding an extra camera based on the ordinary OH PLIF system. The two ICCD cameras combining (315±15) and (360±6) nm bandpass filters were used for OH/kerosene mixed fluorescence and kerosene fluorescence measurement respectively. Then the OH fluorescence of a kerosene Bunsen burner and an engine model kerosene combustion was obtained by subtracting the kerosene from mixed fluorescence pixel by pixel. These results confirmed the feasibility of the kerosene interference elimination method and laid the foundation for the measurement of temperature distribution in kerosene combustion.