In this paper, we developed a photo-counting imaging detector based on the delay-line anode with induction readout, which has the advantages of high sensitivity and large detective area features. This novel detector is expected to be used in space astronomy, bioluminescence and spectral measurement applications. This detector consists of a microchannel plate (MCP) , position-sensitive anode and readout. Among these key parameters, the performance of position-sensitive anode decides the performances of detectors to a large extent. As a charge induction readout delay line anode, the delay line anode decodes the position information of the incident photon by measuring the time delay between two ends of a propagation line. The detector with the anode can obtain high detection sensitivity and a large imaging area. Image charge pickup anode is placed outside the sealed vacuum tube, which not only simplifies the process difficulty of anode production but also improves the detectors reliability. Firstly, An inductive readout delay line anode was designed. We analyzed the influence of different thicknesses and mediums material of the detector on the induction charge of the position-sensitive anode. Then, a method is used to tackle the induction charge of different layers unbalance issue. After that, we designed and fabricated a 40 mm×40 mm position-sensitive anode. The experiment results indicate that the transmission attenuation of the anode output is less than 10%, and the inter-pole crosstalk is less than 3%. Finally, we implemented aphoton-counting imaging experimental system based on this anode. This experimental system provides better than 150um spatial resolution and can promote the theoretical and practical development of large-area array and highly sensitive detector for space astronomical UV spectrum measurement.