In order to promote the research and analysis of panax ginseng proteins through the high quality and resolution 2-DE maps of panax ginseng，a two dimensional electrophoresis technology by gradient polyacrylamide gels is presented. In this work, total sample proteins are firstly extracted from the ginseng root, and then are seperated by IEF and 12.5% humogeneous polyacrylamide gel and 10%~12.5%, 12.5%~15%, 10%~15% gradient polyacrylamide gels. After all images are scaned and analyzed by Image Scanner and Image Master 2D Platinum version 6.0 system, the optional concentration of gradient polyacrylamide gel is obtained. A lot of low abundance protein spots and large molecular weight spots are observed in 10%~15% gradient polyacrylamide gel map, which indicate that the seperation efficiency of gradient gel is highly improved. The results establish the fundation of high quality 2-DE maps of panax ginseng, andprovide a tool for the proteomic analysis of other Araliaceae plants.