The wavefront aberrations of human eye are nor static but fluctuate over time which can cause dynamic changes in both the optical properties and the visual performance of human eyes. The dynamic wavefront aberrations across pupil diameter of 3 mm and 6 mm for 10 eyes with cliopter of 0 D-－5.0 D were measured at temporal frequency of 25 Hz by the modified Hartmann-Shack sensor. Each human eye was tested 125 times in a duration of 5 s. To ensure that measurement did not introduce artifacts, the dynamic wavefront aberrations of 3 mm and 6mm for an artificial eye was acquired. The average amplitude of the fluctuation of the total rms wavefront error of 3mm and 6 mm for 10 eyes is 0.08 μm and 0.105 μm, respectively, which is greater than Marechal diffraction tolerance. The amplitude of the fluctuation of rms wavefront error from 3rd up to 7th order decreases monotonically as the Zernike order increases, the amplitude of the fluctuation are 0.06-0.02 μm. The frequency of the fluctuation for all the Zernike order is 6 Hz.