In order to avoid adverse vibration influence on mobile Doppler wind lidar when it is moving and ensure measurement’s stability and accuracy, a vibration isolation system is designed for cabinets and optical table of mobile wind lidar. SB and M series wire rope isolators of IDC company are respectively selected as the isolators of cabinets and optical table. By calculating, the peak response frequency is less than disturbing frequency and shock displacement is less than the max deflection of the isolators. The relation graph between vibration isolation efficiency and disturbing frequency illustrates that the vibration isolation efficiency is more than 80%. Finally, comparing the locking frequency effect in the same vibration environment before and after vibration isolation system installed, it is obvious that the vibration isolation system improves the frequency stability. It is further certified the reliability of isolation by sine curve fitting of light of sight (LOS) velocity at the elevation of 1 km during moving measurements.