In the present study, the contents of nutritional elements such as Na, K, Ca, Mg, Fe, Zn, Se, Mn and Mo and heavy elements such as Cu, Cd, Pb, Cr and Co of mulberry fruits in three different mature stages were analyzed by ICP-OES. The results showed that the contents of Na, K, Ca, Mg, Fe, Zn, Mn and Cu decreased with maturation development. The contents of the above mentioned mineral elements in green stage were 2.99, 2 957.49, 809.00, 559.95, 36.30, 10.07, 2.12 and 1.32 mg·kg-1, respectively, their contents in pink stage were 169%（Zn）to 14%（Na）lower, the contents of these elements in red stage were 1.63, 2 367.13, 489.30, 221.54, 19.01, 2.64, 0.40 and 0.59 mg·kg-1, and the same changing trend remained in black stage, but the difference was much smaller. The content of Se was the highest in green stage (1.24 mg·kg-1), decreased in pink stage, increased in red stage (0.88 mg·kg-1), but decreased to 0.65 mg·kg-1 in black stage. There was no significant change in Pb content in the first three stages, but a substantial decrease occurred in black stage, and the highest content of Pb was 0.27 mg·kg-1 in green stage. The content of Cr increased during ripening, which was 54.07 mg·kg-1 in red stage, but in black stage it decreased to the same level as that in pink stage. Both the Pb and Cr contents were not over allowance of Chinese National Standard. These data can be well reference for harvesting stage design and evaluation of function and safety of mulberry fruits.