Castanea mollissima Blume has potential as an non-wood forest trees that have been cultivated for thousands of years in China. In order to elucidate the trace elements of chestnut ovary, the major trace elements of self- and cross-pollination chestnut ovary were determined by inductively coupled plasma mass spectrometry. The results showed that self- and cross-pollination 5～50 d, six trace elements trends showed fluctuations. After cross-pollination 20 d, the content of Ca was up to 6.50 mg·g-1, while the self-pollination 10 d, the content of Ca reached up to 7.77 mg·g-1. After cross- and self-pollination pollination 30 d, the content of Mg were highest, 4.19 and 4.69 mg·g-1, respectively. After cross-pollination 5 d, the content of Zn reached the highest, 0.038 7 mg·g-1, while self-pollination 10d the content of Zn was 0.039 9 mg·g-1. After self- and cross-pollination 35 d, the content of Fe were 0.022, 0.019 mg·g-1, respectively. After cross- and self-pollination 20 d, the content of Cu were 0.056, 0.045 mg·g-1, respectively. After self-pollination 40d, the content of Mn reaching the highest was 1.204 mg·g-1, while cross-pollination 30 d, the content of Mn reached its maximum 0.845 mg·g-1. The results can provide a reference for spraying fertilizer on the ovary development, thereby improving chestnut production.